Tissue factor-dependent activation of tritium-labeled factor IX and factor X in human plasma
نویسندگان
چکیده
منابع مشابه
Tissue factor-dependent activation of tritium-labeled factor IX and factor X in human plasma.
Recent investigations have suggested that the activation of factor IX by factor VII/tissue factor may be an important alternative route to the generation of factor Xa. Accordingly, we have compared the tissue factor-dependent activation of tritium-labeled factor IX and factor X in a human plasma system and have studied the role of proteases known to stimulate factor VII activity. Plasma was def...
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15 صفحه اولPurification of Factor VII from bovine plasma. Reaction with tissue factor and activation of Factor X.
Factor VII has been purified up to 330,000-fold from bovine plasma by adsorption on, and elution from, barium sulfate, anion exchange chromatography, gel filtration, and affinity chromatography on benzamidine coupled to Sepharose. The purified material is inhibited by diisopropylphosphorofluoridate both in its native state and after the formation of a complex with tissue factor in the presence ...
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Protease-activated receptor 2 (PAR2) is expressed by vascular endothelial cells and other cells in which its function and physiological activator(s) are unknown. Unlike PAR1, PAR3, and PAR4, PAR2 is not activatable by thrombin. Coagulation factors VIIa (FVIIa) and Xa (FXa) are proteases that act upstream of thrombin in the coagulation cascade and require cofactors to interact with their substra...
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The kinetics of the tissue factor-dependent activation of Factors IX and X have been studied in a bovine plasma system. Heat-defibrinated plasma, which lacks Factors VIII, V, and fibrinogen, was depleted of Factors IX and X by using specific antibodies coupled to Sepharose. Activation initiated by the addition of bovine brain thromboplastin was studied by adding back to the plasma radiolabeled ...
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ژورنال
عنوان ژورنال: Blood
سال: 1984
ISSN: 0006-4971,1528-0020
DOI: 10.1182/blood.v63.6.1338.1338